- Hygienic Equipments
- Biosafety, Purification, Sterilization
Biosafety, Purification, Sterilization
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| BIOSAFETY LEVEL | RISK GROUP | PRACTICE & TECHNIQUES | SAFETY EQUIPMENT | EXAMPLES |
| BL1: Basic | Individual Risk: LOW Community Risk: LOW |
Standard Microbiological Practices. | None: Primary containment provided by adherence to standard lab practices during open bench operations. | E. Coli K12, culture of most non-primate mammalian tissue and cell lines. |
| BL2: Basic with biosafety cabinets and other physical containment devices as required. |
Individual Risk: MODERATE Community Risk: LOW |
Level practices plus: lab coats, autoclaving all biological waste preferred, limited access, bioharzard warning signs on doors and equipment. | Partial containment (i.e., Class I or II biosafety cabinets for procedures which product aerosols.) | Hepatitis B Virus, Salmonella typi, culture of human tumour cell lines, culture of lymphoid lines carrying inducible EBV, manny common human pathogens |
| BL3: Containment with special engineering and design features. |
Individual Risk: HIGH Community Risk: MODERATE |
Level 2 practices plus: special protective clothing, controlled access through entrance room, biological waste must be autoclaved; preferably in facility | Partial containment equipment used for all manipulations of infectious materials, directional airflow. | Yellow fever, M. tuberculosis, short term culture of tissue from non-human primates until cultures are known to be free of Herpes-virus simiae (B. virus) |
| BL4: Maximum Containment | Individual Risk: HIGH Community Risk: HIGH |
Level 3 practices plus: entrance through change room. Complete change of clothing from street to laboratory gear, shower at exit. All wastes decontaminated on exit from facility. | Maximum containment equipment (i.e., Class III biosafety cabinet or partial containment in combination with full-body, air-supplier positive-pressure personnel suit) used for all procedures and activities. | Ebola and Marburg Virus Propagation of Herpes virus simiae (monkey B virus) |
